Supported phospholipid bilayers
نویسندگان
چکیده
منابع مشابه
Protein adsorption on supported phospholipid bilayers.
Quartz crystal microbalance with dissipation (QCM-D) measurements were used to investigate the adsorption of human fibrinogen, human serum albumin, bovine hemoglobin, horse heart cytochrome c, human immunoglobulin (hIgG), and 10% fetal bovine serum on supported bilayers of egg-phosphatidylcholine (eggPC) lipids. For comparison the adsorption of fibrinogen and hIgG to eggPC bilayers was also stu...
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We show that mixing zwitterionic lipids with up to 20% mole % cationic lipids produces gel-phase supported lipid bilayers that are morphologically free of defects detectable using noncontact mode atomic force microscopy (AFM). This contrasts with the observation of massive defects when anionic lipid was added, and also when no charged lipid was added. Infrared measurements of headgroup orientat...
متن کاملPolydopamine-Supported Lipid Bilayers
We report the formation of lipid membranes supported by a soft polymeric cushion of polydopamine. First, 20 nm thick polydopamine films were formed on mica substrates. Atomic force microscopy imaging indicated that these films were also soft with a surface roughness of 2 nm under hydrated conditions. A zwitterionic phospholipid bilayer was then deposited on the polydopamine cushion by fusion of...
متن کاملSlaved diffusion in phospholipid bilayers.
The translational diffusion of phospholipids in supported fluid bilayers splits into two populations when polyelectrolytes adsorb at incomplete surface coverage. Spatially resolved measurements using fluorescence correlation spectroscopy show that a slow mode, whose magnitude scales inversely with the degree of polymerization of the adsorbate, coexists with a fast mode characteristic of naked l...
متن کاملCritical Swelling of Phospholipid Bilayers
We reexamine a critical phenomenon of phospholipids in lamellar phases. The question is, Is the anomalous divergence in the repeat spacing near the main transition the result of a divergence of the water layer or of the lipid bilayer? X-ray diffraction of DLPC lamellae was measured in partially dehydrated conditions. Its critical behavior is much more pronounced than the previously studied DMPC...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1985
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(85)83882-0